The role of ENV genes in extraembryonic mesoderm development
We showed that placenta-derived MSCs expressed endogenous retrovirus gene ERVW1 (syncytin-1). This gene is known to play a key role in trophoblast cells fusion, and, in placental animals, it is necessary for the normal development of the fetus. On the other hand, syncytin-1 is expressed in many other tissues and cells, and even in different types of cancer. The role of this gene in the placenta-derived MSCs is unknown, so we are currently studying the localization of surface (SU) and transmembrane (TM) subunits of syncytin-1 in these cells, as well as the effect of the overexpression and silencing of syncytin-1 on the cell cycle, immunophenotype, transcription profile, and multipotent potential of placenta-derived MSCs. We are also interested in the mechanisms of regulation of ERVW1 expression in placenta-derived MSCs considering the promoter methylation and alternative splicing.
(A) ERVW-1 is colocalized with Golgi apparatus marker TGN38 in PDMCs. Arrows indicate nuclear localization of ERVW-1. (B) ERVW-1 (SU-subunit) was detected in Vim+ stromal cells of chorionic villi of the first trimester of pregnancy. (C) 293T cells were transfected with ERVW1-HA and stained with antibodies against HA-tag. Scale bar: 20 µm. (D) The total cell lysates of PDMCs and BeWo were immunoblotted with monoclonal antibody against the SU subunit of ERVW-1. (E) Relative level of expression of ERVW-1 at protein level.