High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level

Shablii V, Kuchma M, Svitina H, Skrypkina I, Areshkov P, Kyryk V, Bukreieva T, Nikulina V, Shablii Iu, and Lobyntseva G.

BioMed Research International, 2019. https://doi.org/10.1155/2019/2098749

PMCID: PMC6662495

The purpose of this study was to investigate the immunophenotypes and gene expression profile of high proliferative placenta-derived multipotent cells (PDMCs) population at different stages of culture. Our results indicate that PDMCs acquired the representative immunophenotype signature under culture conditions. The subpopulation of PDMC with high proliferation potential and capacity to differentiate in mesodermal directions weakly expressed CK7.

We demonstrated that the colonies resulting from single cells were either positive or negative for CK7, whereas only PDMC clones with weak CK7 expression (CK^lowclones) were highly proliferative

Interestingly, vimentin-positive (Vim⁺) placental stromal mesenchymal cells did not express CK7 in situ. PCNA presence in CK7⁺Vim⁺ cells during placental explants culturing confirmed belonging of these cells to proliferative subpopulation